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NGS Target Capture Seminar and Lunch

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McGill University and Genome Quebec Innovation Center

740 Avenue du Docteur-Penfield, Room 6001

Montréal, QC H3A 0G1

Canada

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Improve uniformity and depth of coverage with xGen® Lockdown® probes

Successful targeted sequencing requires uniform coverage across those sequences. However, sequencing reads are often not distributed evenly over targeted areas. Truncated and/or hard-to-synthesize probes used for target capture often contribute to poor coverage uniformity. Target capture probes, therefore, benefit from quality control and normalization, which is generally not possible for the array-synthesized probes provided by most manufacturers. Thus, many probe pools and panels do not provide uniform coverage.

IDT is uniquely positioned to prepare target capture probes (xGen® Lockdown® Probes) that can provide uniform coverage. IDT Ultramers® are synthesized with a proprietary process, which has the highest base coupling efficiency in the industry. Each oligo is individually synthesized, QC’d and normalized, ensuring that each full-length probe is equally represented in target capture pools. The presence of all full-length probes in equimolar concentration within a pool provides consistent coverage with end-to-end tiling across the target space, thereby improving sequence coverage and uniformity.

Choose from pre-validated panels or design your own custom capture panels:

xGen Exome Research Panel and several other stocked panels available

xGen Predesigned Gene Capture Pools - select a minimum of 10 genes from the exome.

xGen Lockdown Probes – create custom panels or spike-in to existing panels (rescue of drop out from arrays).

About the Speaker: Ashley Dvorak, Field Application Specialist, Integrated DNA Technologies

Ashley joined IDT nearly 10 years ago as part of the Molecular Genetics group. During her tenure, Ashley has held several positions within the company and helped spin off its first Product Development group. She helped research and implement next generation sequencing at IDT, and has assisted in the development and commercialization of IDT’s xGen® Lockdown® product line. Her presentation will provide a technical overview of IDT’s target capture technology alongside comparative data to highlight the distinct advantages that IDT brings to the field of NGS. We encourage questions and welcome the opportunity to discuss your current projects following the presentation.

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McGill University and Genome Quebec Innovation Center

740 Avenue du Docteur-Penfield, Room 6001

Montréal, QC H3A 0G1

Canada

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