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Real-time 0.5-1.8 μl PCR on a Microchip: Nucleic Acid Detection and Identif...

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Location

Location

UBC Point Grey Campus: ETC3 VANCOUVER

3800 Wesbrook Mall

Vancouver, British Columbia V6S 2L9

Canada

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Event description

Description

We invite the Industry and Academia to join us to network and discuss the opportunities and insights that the micro-chip PCR can bring.

10:00-11:00 Registration, welcome coffee & networking

11:00 - 12:00

Microchip Based Real-time PCR Technology: it’s the PCR just like you do it normally, but saving on time and reagent costs

12:00 - 12:15 Coffee-break & Networking

12:15 - 12:45

Demo - Microchip Real-time PCR analyzer AriaDNA: how it is possible to reduce the analysis time and costs

  • Display and short overview about the instrument and its technical specifications
  • Main technical systems of AriaDNA device (thermocycling block and optical system)
  • Basic modes of AriaDNA software operation

12:45 - 14:00 Lunch & Networking

14:00 - 14:30

Demo - Microchips for AriaDNA: how the technology reduces the requirements for the operator qualification

  • Display of liquid PCR reagents and empty microchips
  • Display of freeze-dried PCR reagents and microchips

14:30 - 15:00

Demo - Sample preparation

  • Sample preparation as an important step of PCR analysis that influences PCR results and its importance for microchip real-time PCR
  • DNA/RNA sample preparation: basic principles and practical approaches
  • Demonstration of work-flow using DNA and RNA sample preparation kits and control samples

15:00 - 15:30 Coffee-break & Networking

15:30 - 16:15

Demonstration of the work-flow

  • Using freeze-dried microchips with control samples
  • Using empty microchips with liquid PCR reagents and control samples
  • PCR parameters: thermal cycling mode, detector configuration, microchip template, description of samples
  • Interpretation of results of the analysis, generation of analysis report, importing and exporting the results files.
  • Viewing and understanding the images

16:15 - 17:00 Q&A session

17:00 - 18:00 Networking


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Date and Time

Location

UBC Point Grey Campus: ETC3 VANCOUVER

3800 Wesbrook Mall

Vancouver, British Columbia V6S 2L9

Canada

View Map

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