Scaling Cell Line Development:Enabling IND-Ready, GMP-Compatible Workflows

Generating clonal, edited cell lines with high-precision, audit-ready evidence of clonality – without compromising downstream efficiency – remains a critical challenge in IND-enabling cell line development.

This is especially relevant for stem cell-derived, primary and epithelial cell lines, where optimized single-cell cloning workflows are often lacking. As a result, workflows frequently rely on manual handling, offer limited early visibility and produce fragmented documentation – leading to ambiguous clonality records, repeated subcloning and delays in downstream characterization and tech transfer.

In this webinar, scientists from CCRM/OmniaBio will share their experience applying lessons from hPSC workflows to establish a robust, GMP-compliant epithelial cell line development strategy. By combining gentle single-cell seeding with whole-well, time-stamped imaging from day 0 through colony maturity, the team generated clear evidence of clonality while reducing manual intervention and workflow variability. Media and seeding conditions were optimized to support single-cell survival and outgrowth across challenging cell types.

Importantly, efficient use of clonality data enabled improved identification of edited clones, overcoming limitations of traditional high-throughput screening approaches and extending its value beyond clonality confirmation.

Register for this webinar to learn how early confirmation of clonality can strengthen cell line development, reduce rework and support GMP-aligned workflows.